Early Release – Detection of Highly Pathogenic Avian Influenza A(H5N1) Virus in Cat and Rats during Outbreak in Backyard Poultry, United States, 2025 – Volume 32, Number 10—October 2026 – Emerging Infectious Diseases journal

On March 12, 2025, a farmer in southern Illinois with a small backyard poultry flock brought remains of 6 animals to their primary veterinarian for a necropsy workup after acute illness and near-complete mortality (100/105 birds) of the flock. The farm, ≈2 miles from a 25,000-acre reservoir lake within the Mississippi Flyway, housed 4 turkeys (Meleagris gallopavo), 1 goose (Anser sp.), 20 Guinea fowl (Numida spp.), 80 chickens (Gallus gallus domesticus), 17 cattle, 15 cats, and 2 dogs. Because HPAI virus (HPAIV) was suspected, the 6 animals, a chicken, a turkey, a goose, 2 Guinea fowl, and a rat (Rattus norvegicus), were sent to the University of Missouri Veterinary Medical Diagnostic Laboratory (VMDL; Columbia, MO, USA) for postmortem examination. VMDL collected and evaluated fresh and formalin-fixed tissues and performed influenza A virus (IAV) quantitative reverse transcription PCR (qRT-PCR) on 1 chicken sample, according to guidelines from the US Department of Agriculture (USDA) National Animal Health Laboratory Network (NAHLN; https://www.aphis.usda.gov/labs/nahln). The sample tested presumptive positive per NAHLN guidelines, and VDML subtyped it as H5 influenza. VDML then forwarded the sample to the National Veterinary Services Laboratories (NVSL; Ames, IA, USA) for confirmatory testing, which verified HPAIV H5N1 clade 2.3.4.4b virus.

Further PCR testing of the avian species for Mycoplasma gallisepticum, M. synoviae, and avian paramyxovirus were all negative. The necropsy findings reported hepatitis, pulmonary hemorrhage, and pulmonary edema in the poultry species and bronchopneumonia and meningoencephalitis in the rat. No additional testing was performed on the rat or other bird species. Upon report of HPAIV on the farm, state and federal agencies were notified. The Illinois Department of Agriculture and USDA quarantined the farm and monitored it for 4 months.

On March 16, 2025, the primary veterinarian collected the remains of a 13-year-old domestic shorthaired cat and a second feral rat from the farm. In conjunction with Illinois Department of Agriculture and USDA, the cat and rat specimens were sent to the University of Illinois Veterinary Diagnostic Laboratory (VDL; Urbana, IL, USA) on March 19. VDL performed gross necropsy and collected fresh and formalin-fixed tissues. VDL screened lung tissues from the cat and rat for IAV by qRT-PCR, following NAHLN guidelines. The cat lung tested presumptive positive (cycle threshold [Ct] value 21.2), subtyped as H5; NVSL confirmed H5N1 clade 2.3.4.4b virus. The rat lung tissue was negative upon initial screening, but NVSL conducted follow-up testing on tracheal swab samples, which were H5N1-positive.

Figure 1

Figure 1. Quantitative reverse transcription PCR results from HPAIV A(H5N1) in cat and rats during outbreak in backyard poultry, United States, 2025. Specimens obtained from a cat (A) and a rat (B)…

In addition, we performed follow-up IAV qRT-PCR testing on 12 tissue samples from the cat and 15 tissue samples from the rat and considered Ct values 45 IAV-positive. All 12 cat tissue samples were IAV-positive (Ct values 10.9–31.1), and 11/15 rat tissues were IAV-positive (Ct 17.1–39.4) (Figure 1). Brain tissues of both animals had the highest viral loads (cat Ct 10.9; rat Ct 17.1). Of note, tracheal and nasal swab samples from both animals were IAV-positive (Ct range 22.1–29.5). A second lung sample from the rat returned a Ct of 39.4. Kidney tissues from both species were IAV-positive (cat Ct 20.9; rat Ct 34.6), and urine from the rat also tested IAV-positive (Ct 32.0). Nucleic acid in feces from the cat had a Ct value of 28.6, but no rat feces were available for testing.

Figure 2

Figure 2. Phylogenetic tree of hemagglutinin gene sequences from highly pathogenic avian influenza A(H5N1) virus in cat and rats during outbreak in backyard poultry, United States, 2025. The cat, rat, and chicken…

We performed whole-genome sequencing on 3 samples from the cat (brain, heart, and nasal swab) and 1 brain sample from the rat by using an amplicon-based enrichment protocol and sequencing on a MiSeq platform (Illumina, https://www.illumina.com). We used the Galaxy platform (7) for sequence assembly. All samples returned complete sequences for 8 segments (GenBank accession nos. PX687012–27) (Appendix Table). In addition, we included sequence data of the HPAIV H5N1 strain 25-009367-001 (GenBank accession nos. PV616546–53) from the chicken sample submitted by VDML to NVSL in the analysis. Phylogenetic tree analysis revealed that the chicken, rat, and cat strains clustered together with other avian and mammal HPAIV (Figure 2; Appendix Figures 1–8). All 3 animal species from the outbreak contained the polymerase basic 2 E627K mutation that is associated with mammalian adaptation (8). All cat samples shared identical consensus sequences. We found 2 amino acid mutations, neuraminidase G392D and nucleoprotein K372E, in the cat and rat samples but not in the IAV strain from the chicken. In addition, we noted 1 synonymous polymerase basic 2 C1552T mutation in the rat strain that we did not see in cat and chicken strains. The genotype of the outbreak virus was D1.1 (9) (Appendix).

Figure 3

Figure 3. Histopathology and immunohistochemistry of lesions in a cat and rat infected with highly pathogenic avian influenza A(H5N1) virus during outbreak in backyard poultry, United States, 2025. The cat and rat…

Gross examination during necropsy showed multifocal red mottling of the cat and rat lungs and additional right cranioventral lung consolidation in the rat. Histopathology findings in the cat included multifocal bronchointerstitial pneumonia, mild multifocal nonsuppurative meningoencephalitis, and acute adrenocortical necrosis (Figure 3, panels A–C). We noted severe bronchopneumonia in the rat lung tissue (Figure 3, panel D), likely related to a bacterial infection. Additional lesions in the rat possibly related to viral infection included mild nonsuppurative periventricular encephalitis in brain tissue (Figure 3, panel E) and myocarditis. Immunohistochemistry for IAV in the cat lung showed apical airway epithelial immunoreactivity (Figure 3, panel A, inset), and brain tissue showed multifocal glial and neuronal immunoreactivity (Figure 3, panel C). We also noted mild periventricular immunoreactivity in neurons in the rat brain (Figure 3, panel F).

We describe a strain of H5N1 that resulted in high mortality in a poultry flock and fatal illness in small mammals on a backyard farm in Illinois, USA. Our results show that extensive systemic H5N1 virus spread occurred in both cat and rat tissues after natural infection. Rat nasal turbinates and trachea preferentially expressed α2,3-linked sialic acid receptors relative to lung, likely explaining the lower Ct values in upper respiratory tissues and presence of bacterial bronchopneumonia, potentially leading to increased viral shedding (10).

Cat feces and urine have previously been shown to harbor H5N1 virus (11,12), which our results corroborate. Rats have previously been reported to harbor HPAIV in agricultural settings and have been detected in the current epizootic (13,14). Detection of HPAIV in rat urine and kidney tissue suggests that rodent urine could be a potential transmission medium and warrants further investigation. Rats reside on many farms, can predate birds, and might serve as an intermediate step between infected avian species and susceptible mammalian hosts (15). That coexistence is particularly threatening for commercial poultry and swine confinement farms, which frequently harbor rodent populations despite strict biosecurity efforts. A limitation of our study was that no virus isolation was performed on tissues or urine due to biosafety constraints.

Although cats have been shown to participate in HPAIV transmission, our findings indicate that further investigation is needed to clarify the role of rodents. This study raises awareness of small mammals at the wildlife–livestock interface and highlights the potential role of rats in influenza transmission to commercial livestock production.